Part:BBa_J01080:Experience

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User Reviews

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••••• Antiquity

This review comes from the old result system and indicates that this part worked in some test.

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••• Hong_Kong_HKUST_2014

We saw a strong repression after adding cis-repressing sequence 5’ of the RBS. After arabinose induction to induce the Pbad promoter that regulates the key to the riboregulator system,, we only saw 1.5 fold for 1% arabinose concentration (w/v) induction. For 2.5% we observed a 3 fold increase. The increase, however, only contributed to 0.3% of the fluorescence of the unrepressed controls. For detailed information and figures, visit HKUST iGEM 2014 team wiki page http://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/characterization.

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